Flutey withdrew with a dead leg suffered in training.
But fellow New Zealand born centre Shontayne Hape, a former Kiwi rugby league international who was forced out of his bench place on the morning of the Wales match with a bug, was one of several squad players released on Tuesday along with Leicester midfielder Danny Hipkiss.
England manager Martin Johnson will pick his starting side and bench from the 22-man squad he originally chose for the Wales game plus flanker Chris Robshaw and prop Matt Mullen.
Chris Ashton (Northampton Saints)
Matt Banahan (Bath Rugby)
Jordan Crane (Leicester Tigers)
Shontayne Hape (Bath Rugby)
Dan Hipkiss (Leicester Tigers)
Courtenay Lawes (Northampton Saints)
Lee Mears (Bath Rugby)
Dan Ward-Smith (London Wasps)
Ben Youngs (Leicester Tigers)
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